منابع مشابه
In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae
Vacuole inheritance is temporally coordinated with the cell cycle and is restricted spatially to an axis between the maternal vacuole and the bud. The new bud vacuole is founded by a stream of vacuole-derived membranous vesicles and tubules which are transported from the mother cell into the bud to form the daughter organelle. We now report in vitro formation of vacuole-derived tubules and vesi...
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During budding in Saccharomyces cerevisiae, maternal vacuole material is delivered into the growing daughter cell via tubular or vesicular structures. One of the late steps in vacuole inheritance is the fusion in the bud of vesicles derived from the maternal vacuole. This process has been reconstituted in vitro and requires isolated vacuoles, a physiological temperature, cytosolic factors, and ...
متن کاملThioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae
The vacuole of Saccharomyces cerevisiae projects a stream of tubules a and vesicles (a "segregation structure") into the bud in early S phase. We have described an in vitro reaction, requiring physiological temperature, ATP, and cytosol, in which isolated vacuoles form segregation structures and fuse. This in vitro reaction is defective when reaction components are prepared from vac mutants tha...
متن کاملCooperates with Sec18p (NSF) to Promote Yeast Vacuole Inheritance
Early in S phase, the vacuole (lysosome) of Saccharomyces cerevisiae projects a stream of vesicles and membranous tubules into the bud where they fuse and establish the daughter vacuole. This inheritance reaction can be studied in vitro with isolated vacuoles. Rapid and efficient homotypic fusion between saltwashed vacuoles requires the addition of only two purified soluble proteins, Sec18p (NS...
متن کاملFactoring Mechanism of Reverse Inheritance
In this paper we present a new approach for facilitating the maintenance, reengineering and adaptation of class libraries designed using objectoriented technology. The technique uses a new class relationship called reverse inheritance. We strive to prove that using this class relationship with it’s factoring supporting mechanism it is possible to factor features from a hierarchy, to add new fea...
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ژورنال
عنوان ژورنال: PLANT MORPHOLOGY
سال: 2012
ISSN: 0918-9726,1884-4154
DOI: 10.5685/plmorphol.24.105